Medium data

Search for medium no. = [1344].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1344 SEA WATER MEDIUM WITH ACETATE

NaCl	`20.0`	g
MgCl₂·6H₂O	`3.0`	g
CaCl₂·2H₂O	`0.15`	g
NH₄Cl	`0.25`	g
KH₂PO₄	`0.2`	g
KCl	`0.5`	g
RST trace elements (see Medium No. 933)	`10.0`	ml
Resazurin	`1.0`	mg
Distilled water	`935.0`	ml

Mix components and autoclave under a N₂-CO₂ (4:1, v/v) atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized).

8.0 % NaHCO₃ solution*	`35.0`	ml
RST vitamin solution* (see Medium No. 1097)	`10.0`	ml
1 M Sodium acetate solution	`20.0`	ml
1% Yeast extract solution	`1.0`	ml

Anaerobically and aseptically distribute the medium into appropriate culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N₂-CO₂ (4:1, v/v) gas stream and seal with butyl rubber stoppers. Finally, add per liter the following solution (autoclaved):

Reducing agent solution (see Medium No. 521) 10.0 ml