Medium data
Search for medium no. = [1097].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1097 SEAWATER PYRUVATE THIOSULFATE MEDIUM
| NaCl | 20.0 | g |
| MgCl2·6H2O | 3.0 | g |
| CaCl2·2H2O | 0.15 | g |
| NH4Cl | 0.25 | g |
| KH2PO4 | 0.2 | g |
| KCl | 0.5 | g |
| RST trace elements (see Medium No. 933) | 10.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 922.5 | ml |
Mix components and autoclave under a N2-CO2 (4:1, v/v) atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 37.5 | ml |
| RST vitamin solution* (see below) | 10.0 | ml |
| 1 M Sodium pyruvate solution | 20.0 | ml |
| 1% Yeast extract solution | 10.0 | ml |
Anaerobically and aseptically distribute the medium into appropriate culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Finally, add per liter the following solution:
| Reducing agent solution (see Medium No. 521) | 10.0 | ml |
RST vitamin solution:
| Coenzyme M | 5.0 | mg |
| Biotin | 2.0 | mg |
| Folic acid | 2.0 | mg |
| Pyridoxine·HCl | 10.0 | mg |
| Thiamine·HCl | 5.0 | mg |
| Riboflavin | 5.0 | mg |
| Nicotinic acid | 5.0 | mg |
| DL-Calcium pantothenate | 5.0 | mg |
| p-Aminobenzoic acid | 5.0 | mg |
| Lipoic acid | 5.0 | mg |
| Distilled water | 1.0 | L |
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