Medium data

Search for medium no. = [1097].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1097   SEAWATER PYRUVATE THIOSULFATE MEDIUM

NaCl  20.0     g
MgCl2·6H2O  3.0     g
CaCl2·2H2O  0.15    g
NH4Cl  0.25    g
KH2PO4  0.2     g
KCl  0.5     g
RST trace elements (see Medium No. 933)  10.0     ml
Resazurin  1.0     mg
Distilled water  922.5     ml
Mix components and autoclave under a N2-CO2 (4:1, v/v) atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  37.5     ml
RST vitamin solution* (see below)  10.0     ml
1 M Sodium pyruvate solution  20.0     ml
1% Yeast extract solution  10.0     ml
Anaerobically and aseptically distribute the medium into appropriate culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Finally, add per liter the following solution:
Reducing agent solution (see Medium No. 521)  10.0     ml
RST vitamin solution:
Coenzyme M  5.0     mg
Biotin  2.0     mg
Folic acid  2.0     mg
Pyridoxine·HCl  10.0     mg
Thiamine·HCl  5.0     mg
Riboflavin  5.0     mg
Nicotinic acid  5.0     mg
DL-Calcium pantothenate  5.0     mg
p-Aminobenzoic acid  5.0     mg
Lipoic acid  5.0     mg
Distilled water  1.0     L