Medium data

Search for medium no. = [521].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Mineral medium:
Sea salts (Sigma)  25.0     g
NH4Cl  0.25    g
Na2SO4  3.5     g
Resazurin  1.0     mg
Distilled water  900.0     ml
Mix components and adjust pH to 7.2 - 7.4. Boil Mineral medium and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute Mineral medium into culture vessels (e.g., 9.0 ml of the medium in tubes) under the same gas mixture, seal with Teflon-coated rubber stoppers and autoclave. After cooling, add the following components (per 9.0 ml Mineral medium) from sterile anoxic stock solutions (autoclaved or *filter-sterilized):
8.5% NaHCO3*  0.4     ml
2% Sodium pyruvate solution*  0.5     ml
Trace vitamins (see Medium No. 197)*  0.1     ml
Decane  0.01    ml
1% yeast extract solution  0.05    ml
Reducing agent solution (see below)  0.08    ml
Reducing agent solution:
Na2S·9H2O  12.5     g
L-Cysteine·HCl·H2O  12.5     g
Distilled water  1.0     L
Autoclave and store under a N2 atmosphere.
Comment: For strain JCM 13588, inoculate with a 20% inoculum, and incubate the vessels in inverted position.