Medium data

Search for medium no. = [1341].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1341   MODIFIED PFENNIG MEDIUM WITH ACETATE AND FUMARATE

NaCl  2.0     g
KH2PO4  0.2     g
NH4Cl  0.25    g
MgCl2·6H2O  0.5     g
KCl  0.4     g
CaCl2·2H2O  0.4     g
Resazurin  0.5     mg
Distilled water  920.0     ml
Mix components thoroughly, and autoclave under a N2-CO2 (80:20, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  50.0     ml
1.0 M Sodium fumarate solution  10.0     ml
1.0 M Sodium acetate solution  10.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
Trace element solution* (see Medium No. 899)  1.0     ml
5% Na2S·9H2O solution  6.0     ml
Check pH to be 7.0-7.2.



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