Medium data

Search for medium no. = [1341].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1341 MODIFIED PFENNIG MEDIUM WITH ACETATE AND FUMARATE

NaCl	`2.0`	g
KH₂PO₄	`0.2`	g
NH₄Cl	`0.25`	g
MgCl₂·6H₂O	`0.5`	g
KCl	`0.4`	g
CaCl₂·2H₂O	`0.4`	g
Resazurin	`0.5`	mg
Distilled water	`920.0`	ml

Mix components thoroughly, and autoclave under a N₂-CO₂ (80:20, v/v) gas mixture. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):

8% NaHCO₃ solution*	`50.0`	ml
1.0 M Sodium fumarate solution	`10.0`	ml
1.0 M Sodium acetate solution	`10.0`	ml
Trace vitamins* (see Medium No. 197)	`10.0`	ml
Trace element solution* (see Medium No. 899)	`1.0`	ml
5% Na₂S·9H₂O solution	`6.0`	ml

Check pH to be 7.0-7.2.