Medium data

Search for medium no. = [1338].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1338   PYG MEDIUM (K)

Trypticase peptone  5.0     g
Peptone  5.0     g
Yeast extract  10.0     g
KH2PO4  2.0     g
L-Cysteine·HCl·H2O  0.5     g
Resazurin  1.0     mg
Salt solution (see Medium No. 676)  40.0     ml
Distilled water  940.0     ml
Mix components thoroughly and autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved):
Hemin solution (see Medium No. 469)  10.0     ml
Menadione solution (see Medium No. 469)  10.0     ml
Adjust pH to 7.0-7.2, if necessary.

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