Medium data
Search for medium no. = [1338].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1338 PYG MEDIUM (K)
Trypticase peptone | 5.0 | g |
Peptone | 5.0 | g |
Yeast extract | 10.0 | g |
KH2PO4 | 2.0 | g |
L-Cysteine·HCl·H2O | 0.5 | g |
Resazurin | 1.0 | mg |
Salt solution (see Medium No. 676) | 40.0 | ml |
Distilled water | 940.0 | ml |
Mix components thoroughly and autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved):
Hemin solution (see Medium No. 469) | 10.0 | ml |
Menadione solution (see Medium No. 469) | 10.0 | ml |
Adjust pH to 7.0-7.2, if necessary.
Copyright © 2025 Microbe Division (JCM) - All Rights Reserved