Medium data
Search for medium no. = [1338].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1338 PYG MEDIUM (K)
| Trypticase peptone | 5.0 | g |
| Peptone | 5.0 | g |
| Yeast extract | 10.0 | g |
| KH2PO4 | 2.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
| Resazurin | 1.0 | mg |
| Salt solution (see Medium No. 676) | 40.0 | ml |
| Distilled water | 940.0 | ml |
Mix components thoroughly and autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved):
| Hemin solution (see Medium No. 469) | 10.0 | ml |
| Menadione solution (see Medium No. 469) | 10.0 | ml |
Adjust pH to 7.0-7.2, if necessary.
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