Medium data

Search for medium no. = [469].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

469   TSA HEMIN MENADIONE MEDIUM

Use Medium No. 156 with 10.0 ml/L (final) hemin solution and 10.0 ml/L (final) menadione solution.
Hemin solution:
Dissolve 50 mg hemin first in 1 ml of 1 N NaOH. Then adjust volume to 100 ml.
Menadione solution:
Dissolve 5 mg menadione first in 1 ml of ethanol. Then adjust volume to 100 ml.