Medium data

Search for medium no. = [676].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

676   PYG MEDIUM (G)

Trypticase peptone  5.0     g
Peptone  5.0     g
Yeast extract  10.0     g
Beef extract  5.0     g
Glucose  5.0     g
K2HPO4  2.0     g
L-Cysteine·HCl·H2O  0.5     g
Tween 80  1.0     ml
Resazurin  1.0     mg
Salt solution (see below)  40.0     ml
Distilled water  940.0     ml
Hemin solution (see Medium No. 469)  10.0     ml
Menadione solution (see Medium No. 469)  10.0     ml
Adjust pH to 7.2.
Salt solution:
CaCl2·2H2O  0.25    g
MgSO4·7H2O  0.5     g
K2HPO4  1.0     g
KH2PO4  1.0     g
NaHCO3  10.0     g
NaCl  2.0     g
Distilled water  1.0     L