Medium data

Search for medium no. = [1313].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1313 MODIFIED AM5 MEDIUM 2

NaCl	`1.0`	g
KCl	`0.5`	g
MgCl₂·6H₂O	`0.4`	g
CaCl₂·2H₂O	`0.1`	g
NH₄Cl	`0.3`	g
KH₂PO₄	`0.2`	g
L-Cysteine·HCl·H₂O	`0.2`	g
Resazurin	`0.4`	mg
Distilled water	`910.0`	ml

Mix components thoroughly and autoclave under a N₂-CO₂ (9:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):

8% NaHCO₃ solution*	`40.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Selenite tungstate solution (see Medium No. 431)	`1.0`	ml
Trace vitamins (see Medium No. 197)*	`10.0`	ml
Vitamin B₁₂ solution (see Medium No. 403)*	`1.0`	ml
Aromatic fatty acids solution (see Medium No. 1051)	`1.0`	ml
Menadione solution (see below)	`1.0`	ml
Fatty acid mixture (see Medium No. 266)*	`1.0`	ml
10% Yeast extract solution*	`10.0`	ml
10% Casamino acids solution*	`10.0`	ml
1 M Glucose solution	`5.0`	ml
0.1 M Dithiothreitol solutuion*	`10.0`	ml

Adjust pH to 7.5, if necessary.
Menadione solution:
Autoclave 100 mg menadione (vitamin K₃) with 100 ml 5% NaHCO₃ solution. Remove insoluble residue by filtration. Store the solution under a N₂-CO₂ (8:2, v/v) gas mixture.