Medium data

Search for medium no. = [1313].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1313   MODIFIED AM5 MEDIUM 2

NaCl  1.0     g
KCl  0.5     g
MgCl2·6H2O  0.4     g
CaCl2·2H2O  0.1     g
NH4Cl  0.3     g
KH2PO4  0.2     g
L-Cysteine·HCl·H2O  0.2     g
Resazurin  0.4     mg
Distilled water  910.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (9:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  40.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
FeCl2 solution (see Medium No. 187)  1.0     ml
Selenite tungstate solution (see Medium No. 431)  1.0     ml
Trace vitamins (see Medium No. 197)*  10.0     ml
Vitamin B12 solution (see Medium No. 403)*  1.0     ml
Aromatic fatty acids solution (see Medium No. 1051)  1.0     ml
Menadione solution (see below)  1.0     ml
Fatty acid mixture (see Medium No. 266)*  1.0     ml
10% Yeast extract solution*  10.0     ml
10% Casamino acids solution*  10.0     ml
1 M Glucose solution  5.0     ml
0.1 M Dithiothreitol solutuion*  10.0     ml
Adjust pH to 7.5, if necessary.
Menadione solution:
Autoclave 100 mg menadione (vitamin K3) with 100 ml 5% NaHCO3 solution. Remove insoluble residue by filtration. Store the solution under a N2-CO2 (8:2, v/v) gas mixture.



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