Medium data

Search for medium no. = [1302].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1302 SEA SALTS YTG MEDIUM

Yeast extract	`0.5`	g
Tryptone	`1.0`	g
Glucose	`2.5`	g
Sea salts (Sigma-Aldrich)	`30.0`	g
Trace minerals (see Medium No. 151)	`1.0`	ml
0.1% Na₂SeO₃ solution	`1.0`	ml
L-Cysteine·HCl·H₂O	`0.2`	g
Resazurin	`0.5`	mg
Distilled water	`1.0`	L

Mix components and adjust pH to 7.0. Dispense the medium into culture vessels under a N₂ gas atmosphere (e.g., 10 ml medium in Hungate tubes), seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobicallly add per liter the following solutions (filter-sterilized):

Trace vitamins (see Medium No. 197)	`10.0`	ml
1M Sodium thiosulfate solution	`5.0`	ml