Medium data

Search for medium no. = [1294].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1294 MMJ/KT2 MEDIUM

Modified MJ synthetic seawater C	`1.0`	L
Resazurin	`1.0`	mg

Adjust pH to 7.0 and autoclave. After cooling, add the following solutions (filter-sterilized):

8% NaHCO₃ solution	`12.5`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml
Vitamin B₁₂ solution (see Medium No. 403)	`0.2`	ml

Aseptically distribute the medium into culture vessels under a stream of H₂-CO₂ (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under N₂):

5% L-Cysteine·HCl·H₂O solution	`10.0`	ml
5% Na₂S·9H₂O solution	`10.0`	ml

Pressurize the inoculated bottles to 200 kPa H₂-CO₂ (4:1, v/v).
Modified MJ synthetic seawater C:

NaCl	`25.0`	g
MgCl₂·6H₂O	`4.2`	g
MgSO₄·7H₂O	`3.4`	g
KCl	`0.5`	g
NH₄Cl	`0.25`	g
K₂HPO₄	`0.14`	g
CaCl₂·2H₂O	`0.7`	g
FeSO₄·7H₂O	`0.02`	g
Trace mineral solution (see below)	`10.0`	ml
Distilled water	`1.0`	L

Trace mineral solution:

Trace minerals (see Medium No. 151)	`1.0`	L
NiCl₂·6H₂O	`0.075`	g
Na₂SeO₃·5H₂O	`0.05`	g
Na₂WO₄·2H₂O	`0.1`	g