Medium data

Search for medium no. = [1294].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1294   MMJ/KT2 MEDIUM

Modified MJ synthetic seawater C  1.0     L
Resazurin  1.0     mg
Adjust pH to 7.0 and autoclave. After cooling, add the following solutions (filter-sterilized):
8% NaHCO3 solution  12.5     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Vitamin B12 solution (see Medium No. 403)  0.2     ml
Aseptically distribute the medium into culture vessels under a stream of H2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under N2):
5% L-Cysteine·HCl·H2O solution  10.0     ml
5% Na2S·9H2O solution  10.0     ml
Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
Modified MJ synthetic seawater C:
NaCl  25.0     g
MgCl2·6H2O  4.2     g
MgSO4·7H2O  3.4     g
KCl  0.5     g
NH4Cl  0.25    g
K2HPO4  0.14    g
CaCl2·2H2O  0.7     g
FeSO4·7H2O  0.02    g
Trace mineral solution (see below)  10.0     ml
Distilled water  1.0     L
Trace mineral solution:
Trace minerals (see Medium No. 151)  1.0     L
NiCl2·6H2O  0.075   g
Na2SeO3·5H2O  0.05    g
Na2WO4·2H2O  0.1     g



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