Medium data

Search for medium no. = [1290].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1290 NRmbB1 MEDIUM

NH₄Cl	`0.54`	g
KH₂PO₄	`0.14`	g
MgCl₂·6H₂O	`3.0`	g
CaCl₂·2H₂O	`0.15`	g
NaCl	`20.0`	g
Trace mineral solution (see Medium No. 852)	`1.0`	ml
Se/W solution (see Medium No. 852)	`1.0`	ml
NaHCO₃	`2.5`	g
Resazurin	`1.0`	mg
Distilled water	`900.0`	ml

Mix components except NaHCO₃ and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N₂-CO₂ (80:20, v/v) and add NaHCO₃ to the solution. Dispense the medium into culture vessels under a stream of N₂-CO₂ (80:20, v/v), seal with butyl rubber stopper and autoclave. After autoclaving, stand the medium overnight. Aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):

Vitamin solution* (see Medium No. 197)	`1.0`	ml
Vitamin B₁₂ solution* (see Medium No. 403)	`0.1`	ml
1.0 M Glucose solution	`20.0`	ml
0.5 M Coenzyme M solution	`10.0`	ml
5% Na₂S·9H₂O solution	`6.0`	ml