Medium data

Search for medium no. = [1290].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1290   NRmbB1 MEDIUM

NH4Cl  0.54    g
KH2PO4  0.14    g
MgCl2·6H2O  3.0     g
CaCl2·2H2O  0.15    g
NaCl  20.0     g
Trace mineral solution (see Medium No. 852)  1.0     ml
Se/W solution (see Medium No. 852)  1.0     ml
NaHCO3  2.5     g
Resazurin  1.0     mg
Distilled water  900.0     ml
Mix components except NaHCO3 and adjust pH to 6.5. Bring to a boil for 5-10 sec, then, cool down under a stream of N2-CO2 (80:20, v/v) and add NaHCO3 to the solution. Dispense the medium into culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stopper and autoclave. After autoclaving, stand the medium overnight. Aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
Vitamin solution* (see Medium No. 197)  1.0     ml
Vitamin B12 solution* (see Medium No. 403)  0.1     ml
1.0 M Glucose solution  20.0     ml
0.5 M Coenzyme M solution  10.0     ml
5% Na2S·9H2O solution  6.0     ml