Medium data

Search for medium no. = [1277].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1277 VULCANO HIGH SALT MEDIUM

NaCl	`18.0`	g
MgCl₂·6H₂O	`1.0`	g
MgSO₄·7H₂O	`1.0`	g
KCl	`0.3`	g
Na₂SO₄	`1.0`	g
(NH₄)₂SO₄	`0.3`	g
K₂HPO₄	`0.05`	g
KH₂PO₄	`0.05`	g
CaCl₂·2H₂O	`0.02`	g
FeCl₂·4H₂O	`0.1`	g
Iron(III) citrate	`0.1`	g
L-Cysteine·HCl·H₂O	`0.5`	g
MES	`1.95`	g
Resazurin	`1.0`	mg
Trace element solution (see Medium No. 439)	`1.0`	ml
Se-Ni-W solution (see Medium No. 1243)	`1.0`	ml
Distilled water	`1.0`	L

Mix components thoroughly, adjust pH to 6.3 and autoclave under a N₂-CO₂ (4:1, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved):

10% Yeast extract solution	`5.0`	ml
1.0 M Sodium fumarate solution	`10.0`	ml

Comment:
For JCM 39183, add 1.0 M glucose solution (final 10 mM), instead of sodium fumarate solution, and adjust pH to 5.5.