Medium data
Search for medium no. = [1243].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1243 VULCANO LOW SALT MEDIUM
| NaCl | 1.7 | g |
| MgCl2·6H2O | 1.0 | g |
| MgSO4·7H2O | 1.0 | g |
| KCl | 0.3 | g |
| Na2SO4 | 1.0 | g |
| (NH4)2SO4 | 0.3 | g |
| K2HPO4 | 0.05 | g |
| KH2PO4 | 0.05 | g |
| CaCl2·2H2O | 0.02 | g |
| FeCl2·4H2O | 0.1 | g |
| Iron(III) citrate | 0.1 | g |
| L-Cysteine·HCl·H2O | 0.3 | g |
| Resazurin | 1.0 | mg |
| Trace element solution (see Medium No. 439) | 1.0 | ml |
| Se-Ni-W solution (see below) | 1.0 | ml |
| Distilled water | 966.0 | ml |
Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved):
| 10% Yeast extract solution | 5.0 | ml |
| 10% Trypticase peptone solution | 5.0 | ml |
| 1.0 M Sodium acetate solution | 2.0 | ml |
Adjust pH to 3.8-4.0 with HCl.
Se-Ni-W solution:
| Na2SeO3 | 10.0 | mg |
| NiCl2·6H2O | 20.0 | mg |
| Na2WO4·2H2O | 30.0 | mg |
| Distilled water | 100.0 | ml |
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