Medium data

Search for medium no. = [1256].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1256   DESULFOCELLA MEDIUM

Na2SO4  3.0     g
KH2PO4  0.2     g
NH4Cl  0.3     g
NaCl  21.0     g
MgCl2·6H2O  3.0     g
KCl  0.5     g
CaCl2·2H2O  0.15    g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
Resazurin  0.5     mg
Distilled water  1.0     L
Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 10 ml in Balch tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 gas atmosphere or *filter-sterilized and stored under an 80% N2-20% CO2 gas mixture):
Trace vitamins* (see Medium No. 197)  10.0     ml
Substrate solution (see below)  10.0     ml
8% NaHCO3 solution*  20.0     ml
5% Na2S·9H2O solution  8.0     ml
Substrate solution:
Sodium butyrate  0.7     g
Sodium caproate  0.3     g
Sodium octanoate  0.15    g
Distilled water  10.0     ml