Medium data

Search for medium no. = [1256].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1256 DESULFOCELLA MEDIUM

Na₂SO₄	`3.0`	g
KH₂PO₄	`0.2`	g
NH₄Cl	`0.3`	g
NaCl	`21.0`	g
MgCl₂·6H₂O	`3.0`	g
KCl	`0.5`	g
CaCl₂·2H₂O	`0.15`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
Resazurin	`0.5`	mg
Distilled water	`1.0`	L

Mix components thoroughly, bring to a boil and cool down under a N₂-CO₂ (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 10 ml in Balch tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N₂ gas atmosphere or *filter-sterilized and stored under an 80% N₂-20% CO₂ gas mixture):

Trace vitamins* (see Medium No. 197)	`10.0`	ml
Substrate solution (see below)	`10.0`	ml
8% NaHCO₃ solution*	`20.0`	ml
5% Na₂S·9H₂O solution	`8.0`	ml

Substrate solution:

Sodium butyrate	`0.7`	g
Sodium caproate	`0.3`	g
Sodium octanoate	`0.15`	g
Distilled water	`10.0`	ml