Medium data

Search for medium no. = [1245].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NaCl  1.0     g
MgCl2·6H2O  0.2     g
NaH2PO4·2H2O  0.91    g
CaCl2·2H2O  0.1     g
NH4Cl  1.0     g
Yeast extract (BD-Difco)  0.2     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Distilled water  900.0     ml
Mix components thoroughly and adjust pH to 7.2. Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a stream of N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Allow to stand overnight. Aseptically and anaerobically add the following solutions (filter-sterilized):
8.0% NaHCO3 solution  30.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
0.25 M Lactose solution  50.0     ml
Readjust pH to 7.2-7.6, if necessary.

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