Medium data
Search for medium no. = [1245].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1245 TRICHOCOCCUS SKBG MEDIUM
| NaCl | 1.0 | g |
| MgCl2·6H2O | 0.2 | g |
| NaH2PO4·2H2O | 0.91 | g |
| CaCl2·2H2O | 0.1 | g |
| NH4Cl | 1.0 | g |
| Yeast extract (BD-Difco) | 0.2 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Distilled water | 900.0 | ml |
Mix components thoroughly and adjust pH to 7.2. Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a stream of N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Allow to stand overnight. Aseptically and anaerobically add the following solutions (filter-sterilized):
| 8.0% NaHCO3 solution | 30.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| 0.25 M Lactose solution | 50.0 | ml |
Readjust pH to 7.2-7.6, if necessary.
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