Medium data
Search for medium no. = [1228].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1228 ANAEROBIC ALKALINE GLUCOSE MEDIUM
Solution A:
| NaCl | 20.0 | g |
| Na2CO3 | 50.0 | g |
| NaHCO3 | 60.0 | g |
| Distilled water | 900.0 | ml |
Mix components thoroughly and autoclave under a N2 gas atmosphere.
Solution B:
| MgCl2·6H2O | 0.1 | g |
| NH4Cl | 0.5 | g |
| KH2PO4 | 0.2 | g |
| Yeast extract | 0.05 | g |
| Glucose | 3.0 | g |
| Trace element solution (see Medium No. 899) | 1.0 | ml |
| Distilled water | 100.0 | ml |
Mix components thoroughly and autoclave under a N2 gas atmosphere.
Aseptically and anaerobically combine Solutions A and B. Check final pH of the medium to be around 9.2. Add the following solution into the medium (filter-sterilized and stored under a N2 atmosphere):
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
Comment: For JCM 32929, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
| 10% Peptone solution | 10.0 | ml |
| 5% Na2S·9H2O solution | 6.0 | ml |
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