Medium data
Search for medium no. = [1218].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1218 MODIFIED METHANOHALOPHILUS MEDIUM
| NaCl | 120.0 | g |
| KCl | 1.5 | g |
| MgCl2·6H2O | 6.0 | g |
| CaCl2·2H2O | 0.4 | g |
| NH4Cl | 1.0 | g |
| K2HPO4 | 0.30 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Yeast extract (Oxoid) | 2.0 | g |
| Trypticase peptone (BD-BBL) | 2.0 | g |
| Resazurin | 0.5 | mg |
| Distilled water | 920.0 | ml |
Mix components thoroughly and autoclave the medium under a N2:CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions (filter-sterilized):
| 8% NaHCO3 solution | 50.0 | ml |
| 1.4% Coenzyme M solution | 10.0 | ml |
| 1 M Trimethylamine·HCl solution | 20.0 | ml |
Adjust pH to 7.0-7.2, if necessary. Aseptically and anaerobically, distribute the medium into culture vessels under the same gas mixture (e.g., 20 ml medium in 60 ml-serum bottles) and seal with butyl rubber stoppers. Add per liter the following solution (autoclaved and stored under a N2 atmosphere).
| 5% Na2S·9H2O solution | 5.0 | ml |
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