Medium data

Search for medium no. = [1218].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NaCl  120.0     g
KCl  1.5     g
MgCl2·6H2O  6.0     g
CaCl2·2H2O  0.4     g
NH4Cl  1.0     g
K2HPO4  0.30    g
Trace minerals (see Medium No. 151)  10.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Yeast extract (Oxoid)  2.0     g
Trypticase peptone (BD-BBL)  2.0     g
Resazurin  0.5     mg
Distilled water  920.0     ml
Mix components thoroughly and autoclave the medium under a N2:CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions (filter-sterilized):
8% NaHCO3 solution  50.0     ml
1.4% Coenzyme M solution  10.0     ml
1 M Trimethylamine·HCl solution  20.0     ml
Adjust pH to 7.0-7.2, if necessary. Aseptically and anaerobically, distribute the medium into culture vessels under the same gas mixture (e.g., 20 ml medium in 60 ml-serum bottles) and seal with butyl rubber stoppers. Add per liter the following solution (autoclaved and stored under a N2 atmosphere).
5% Na2S·9H2O solution  5.0     ml

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