Medium data
Search for medium no. = [1214].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1214 RECTINEMA SUBTERRANEUM MEDIUM
| Major salts solution (see below) | 100.0 | ml |
| NaCl | 10.0 | g |
| Peptone | 1.0 | g |
| Yeast extract | 1.0 | g |
| HEPES | 1.0 | g |
| Trace minerals (see Medium No. 151) | 20.0 | ml |
| Trace vitamins (see Medium No. 197) | 20.0 | ml |
| Distilled water | 860.0 | ml |
Mix components thoroughly, adjust pH to 7.2 and autoclave under a H2-CO2 (80:20, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solution (autoclaved and stored under N2):
| 5% Na2S·9H2O solution | 10.0 | ml |
Major salts solution:
| KCl | 0.12 | g |
| MgSO4·7H2O | 0.3 | g |
| CaSO4·2H2O | 0.076 | g |
| Na2SO4 | 0.4 | g |
| CaCl2·2H2O | 0.024 | g |
| NH4Cl | 0.25 | g |
| (NH4)2SO4 | 0.32 | g |
| KH2PO4 | 0.45 | g |
| NaF | 0.06 | g |
| LiCl | 0.02 | g |
| Distilled water | 1.0 | L |
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved