Medium data

Search for medium no. = [1194].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1194   METHANOTHERMOCOCCUS HHB MEDIUM

NH4Cl  0.5     g
K2HPO4  0.14    g
KCl  0.33    g
MgCl2·6H2O  4.18    g
MgSO4·7H2O  3.4     g
CaCl2·2H2O  0.14    g
NaCl  20.0     g
Fe(NH4)2(SO4)2·6H2O  2.0     mg
Trace vitamins (see Medium No. 197)  10.0     ml
Trace mineral solution (see Medium No. 852)  10.0     ml
Se/W solution (Medium No. 852)  1.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly. Bring to a boil and cool down under a H2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture vessels (e.g., 20 ml medium in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  12.0     ml
5% Na2S·9H2O solution  10.0     ml
Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).