Medium data

Search for medium no. = [1181].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1181   MODIFIED CELLULOLYTIC HALOARCHAEA MEDIUM

MDS salt water (see Medium No. 574)  833.0     ml
Trace element solution (see Medium No. 1079)  1.0     ml
Yeast extract (BD-Difco)  0.1     g
Add components and bring volume to 958 ml with distilled water. For preparation of solid medium, add 20 g/L agar (if necessary, use washed agar: see Medium No. 574). Autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized) to the medium.
1 M NH4Cl  5.0     ml
Potassium phosphate buffer (see Medium No. 574)  2.0     ml
Trace vitamins* (see Medium No. 197)  5.0     ml
0.2 M Cellobiose solution  30.0     ml
Readjust pH to 7.0 with a sterilized 10% Na2CO3 solution, if necessary.



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