Medium data

Search for medium no. = [1180].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1180   CELLULOLYTIC NATRONOARCHAEA MEDIUM

NaCl  128.0     g
Na2CO3  95.0     g
NaHCO3  15.0     g
KCl  2.5     g
K2HPO4  1.75    g
Add components to distilled water and bring volume to 1.0 L. After cooling, add the following solutions (autoclaved or *filter-sterilized):
10% NH4Cl solution  1.25    ml
1 M MgSO4 solution  1.0     ml
Trace element solution (see Medium No. 1079)  1.0     ml
Trace vitamins* (see Medium No. 197)  5.0     ml
10% Yeast extract solution  0.2     ml
10% Cellobiose solution  20.0     ml