Medium data

Search for medium no. = [1177].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1177   SEDIMENTISPHAERA L21 MEDIUM

NaCl  120.0     g
MgCl2·6H2O  6.0     g
KCl  1.5     g
Na2SO4  1.0     g
NH4Cl  1.0     g
CaCl2·2H2O  0.4     g
K2HPO4  0.4     g
Trace mineral solution (see Medium No. 1084)  10.0     ml
Resazurin  0.5     mg
Distilled water  950.0     ml
Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream and autoclave under the same gas mixture. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized):
10% Glucose solution  10.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
8% NaHCO3 solution*  30.0     ml
Aseptically and anaerobically, dispense the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
5% L-Cysteine·HCl·H2O solution  10.0     ml
5% Na2S·9H2O solution  10.0     ml
Readjust pH to 7.5, if necessary.