Medium data
Search for medium no. = [1084].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1084 THIOBACILLUS PROSPERUS MEDIUM
| KCl | 0.33 | g |
| MgCl2·6H2O | 2.75 | g |
| MgSO4·7H2O | 3.45 | g |
| NH4Cl | 1.25 | g |
| CaCl2·2H2O | 0.14 | g |
| KH2PO4 | 0.14 | g |
| K2HPO4 | 0.14 | g |
| NaCl | 0.5 | g |
| NiCl2·6H2O | 2.0 | mg |
| Trace mineral solution (see below) | 10.0 | ml |
| Distilled water | 940.0 | ml |
Adjust pH to 2.5 and autoclave. After cooling, aseptically add the following solution (filter-sterilized):
| FeSO4·7H2O solution (see below) | 50.0 | ml |
Trace mineral solution:
| Trace minerals (see Medium No. 151) | 1.0 | L |
| NiCl2·6H2O | 0.03 | g |
| Na2SeO3·5H2O | 0.3 | mg |
| NaWO4·2H2O | 0.4 | mg |
FeSO4·7H2O solution:
Dissolve 40.0 g FeSO4·7H2O in 0.1 N H2SO4 solution and fill up to 100.0 ml. Filter-sterilize and store under a N2 atmosphere.
Copyright © 2024 Microbe Division (JCM) - All Rights Reserved