Medium data

Search for medium no. = [1176].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1176 METHANOSARCINA JL01 MEDIUM

KH₂PO₄	`0.15`	g
K₂HPO₄	`0.29`	g
NH₄Cl	`1.0`	g
MgCl₂·6H₂O	`0.2`	g
CaCl₂·2H₂O	`0.1`	g
NaCl	`0.9`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Yeast extract (Oxoid)	`2.0`	g
Resazurin	`0.5`	mg
NaHCO₃	`2.0`	g
Distilled water	`1.0`	L

Mix components except NaHCO₃ and bring to a boil for a few seconds. After cooling down under a stream of N₂-CO₂ (80:20, v/v), add NaHCO₃ to the medium. Dispense the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Allow to stand the medium until re-dissolved. Then, add per liter the following solutions (autoclaved or *filter-sterilized):

Trace vitamins* (see Medium No. 197)	`10.0`	ml
50% (v/v) Methanol	`10.0`	ml
5% L-Cysteine·HCl·H₂O solution	`10.0`	ml
5% Na₂S·9H₂O solution	`10.0`	ml

Adjust pH of the medium to 6.8-7.0, if necessary.