Medium data

Search for medium no. = [1176].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1176   METHANOSARCINA JL01 MEDIUM

KH2PO4  0.15    g
K2HPO4  0.29    g
NH4Cl  1.0     g
MgCl2·2H2O  0.2     g
CaCl2·2H2O  0.1     g
NaCl  0.9     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Yeast extract (Oxoid)  2.0     g
Resazurin  0.5     mg
NaHCO3  2.0     g
Distilled water  1.0     L
Mix components except NaHCO3 and bring to a boil for a few seconds. After cooling down under a stream of N2-CO2 (80:20, v/v), add NaHCO3 to the medium. Dispense the medium into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Allow to stand the medium until re-dissolved. Then, add per liter the following solutions (autoclaved or *filter-sterilized):
Trace vitamins* (see Medium No. 197)  10.0     ml
50% (v/v) Methanol  10.0     ml
5% L-Cysteine·HCl·H2O solution  10.0     ml
5% Na2S·9H2O solution  10.0     ml
Adjust pH of the medium to 6.8-7.0, if necessary.