Medium data

Search for medium no. = [1175].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1175 CAENICOLA MOBILE MEDIUM

KH₂PO₄	`0.5`	g
K₂HPO₄	`0.5`	g
NH₄Cl	`0.5`	g
NaCl	`0.4`	g
MgCl₂·6H₂O	`0.3`	g
CaCl₂·2H₂O	`0.05`	g
KCl	`0.4`	g
Yeast extract	`2.0`	g
Tryptone (BD-Difco)	`2.0`	g
Casamino acids (BD-Difco)	`2.0`	g
L-Cysteine	`2.4`	g
L-Arginine	`4.2`	g
L-Histidine	`4.2`	g
Trace metal solution (see Medium No. 294)	`1.0`	ml
Resazurin	`1.0`	mg
Distilled water	`975.0`	ml

Mix components thoroughly, adjust pH to 7.5 with KOH and autoclave under a N₂-CO₂ (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized):

8% NaHCO₃ solution	`25.0`	ml
Trace vitamins (see Medium No. 197)	`1.0`	ml

Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N₂-CO₂ (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add per liter the following solution (autoclaved and stored under N₂):

5% Na₂S·9H₂O solution 8.0 ml

Readjust pH to 7.5, if necessary.