Medium data

Search for medium no. = [1162].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1162 NAS-02 MEDIUM

(NH₄)₂SO₄	`0.2`	g
KH₂PO₄	`3.0`	g
Modified Allen's trace metal solution(see Medium No. 1014)	`1.0`	ml
Na₂S₂O₃·5H₂O	`2.5`	g
Distilled water	`1.0`	L

Mix components thoroughly and adjust pH to 5.2. Autoclave under a N₂-CO₂ (4:1, v/v) gas mixture. After cooling, add the following solutions anaerobically and aseptically (autoclaved or *filter-sterilized and store under a N₂ atmosphere):

5% (w/v) MgSO₄·7H₂O solution	`10.0`	ml
5% (w/v) CaCl₂·2H₂O solution	`5.0`	ml
Trace vitamins* (see Medium No. 197)	`10.0`	ml
10% (w/v) Yeast extract solution	`5.0`	ml
25 mM L-Cysteine·HCl solution	`10.0`	ml
130 mM FeCl₂ solution	`10.0`	ml