Medium data

Search for medium no. = [1157].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NaCl  5.0     g
KH2PO4  0.2     g
Na2SO4  4.0     g
Na2CO3  3.5     g
NH4Cl  0.25    g
MgCl2·6H2O  0.1     g
KCl  0.2     g
Yeast extract  0.2     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Fe-Ni-WO-Se solution (see Medium No. 1121)  1.0     ml
Distilled water  1.0     L
Mix components thoroughly and adjust pH to 9.0. Bring to a boil and cool under a N2 gas stream. Distribute the medium into culture vessels (e.g., 10 ml of the medium in the Hungate tubes) under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
Vitamin solution* (see below)  1.0     ml
1 M Sodium lactate solution  20.0     ml
5% Na2S·9H2O solution  8.0     ml
Vitamin solution:
Thiamine·HCl  10.0     mg
Nicotinic acid  20.0     mg
DL-Calcium pantothenate  5.0     mg
Pyridoxine·HCl  50.0     mg
Biotin  2.0     mg
p-Aminobenzoic acid  10.0     mg
Vitamin B12  20.0     mg
Distilled water  1.0     L