Medium data
Search for medium no. = [1157].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1157 DESULFONATRONUM PAR180 MEDIUM
| NaCl | 5.0 | g |
| KH2PO4 | 0.2 | g |
| Na2SO4 | 4.0 | g |
| Na2CO3 | 3.5 | g |
| NH4Cl | 0.25 | g |
| MgCl2·6H2O | 0.1 | g |
| KCl | 0.2 | g |
| Yeast extract | 0.2 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Fe-Ni-WO-Se solution (see Medium No. 1121) | 1.0 | ml |
| Distilled water | 1.0 | L |
Mix components thoroughly and adjust pH to 9.0. Bring to a boil and cool under a N2 gas stream. Distribute the medium into culture vessels (e.g., 10 ml of the medium in the Hungate tubes) under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere):
| Vitamin solution* (see below) | 1.0 | ml |
| 1 M Sodium lactate solution | 20.0 | ml |
| 5% Na2S·9H2O solution | 8.0 | ml |
Vitamin solution:
| Thiamine·HCl | 10.0 | mg |
| Nicotinic acid | 20.0 | mg |
| DL-Calcium pantothenate | 5.0 | mg |
| Pyridoxine·HCl | 50.0 | mg |
| Biotin | 2.0 | mg |
| p-Aminobenzoic acid | 10.0 | mg |
| Vitamin B12 | 20.0 | mg |
| Distilled water | 1.0 | L |
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