Medium data

Search for medium no. = [1121].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1121 AZC 1502 MEDIUM

KH₂PO₄	`0.1`	g
NH₄Cl	`0.1`	g
KCl	`0.05`	g
CaCl₂·2H₂O	`0.05`	g
MgCl₂·6H₂O	`0.1`	g
Na₂S₂O₃·5H₂O	`3.2`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Fe-Ni-WO-Se solution (see below)	`1.0`	ml
Distilled water	`1.0`	L

Mix components thoroughly and adjust pH to 5.0. Bring to a boil and cool under a N₂ gas stream. Distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under the same gas atmosphere, seal with butyl rubber stoppers, and autoclave. After cooling, replace the gas phase with a H₂-CO₂ (4:1, v/v) gas mixture and add per liter the following solution (freshly prepared and filter-sterilized):

1 M Sodium acetate solution	`10.0`	ml
1% Yeast extract solution	`10.0`	ml
0.1% Sodium dithionite solution	`20.0`	ml

Pressurize the inoculated culture vessels to 100 kPa H₂-CO₂ (4:1, v/v).
Fe-Ni-WO-Se solution:

FeSO₄·7H₂O	`1.42`	g
NiSO₄·7H₂O	`1.6`	g
Na₂WO₄·2H₂O	`38.0`	mg
Na₂SeO₃·5H₂O	`3.0`	mg
Distilled water	`1.0`	L