Medium data

Search for medium no. = [1155].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1155 DESULFOOBULBUS OLIGOTROPHICUS MEDIUM

KH₂PO₄	`0.5`	g
K₂HPO₄	`0.5`	g
NH₄Cl	`0.5`	g
NaCl	`5.0`	g
Na₂SO₄	`2.84`	g
MgCl₂·6H₂O	`0.3`	g
CaCl₂·2H₂O	`0.05`	g
KCl	`0.4`	g
Trace element solution (see Medium No. 294)	`1.0`	ml
Resazurin	`1.0`	mg
Distilled water	`955.0`	ml

Mix components thoroughly, adjust pH to 7.6 with KOH and autoclave under a N₂-CO₂ (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter-sterilized):

8% NaHCO₃ solution*	`25.0`	ml
1 M Sodium propionate solution	`20.0`	ml
Trace vitamins* (see Medium No. 197)	`1.0`	ml

Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N₂-CO₂ (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add per liter the following solution (autoclaved and stored under N₂):

5% Na₂S·9H₂O solution 8.0 ml

Readjust pH to 7.6, if necessary.