Medium data

Search for medium no. = [1155].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1155   DESULFOOBULBUS OLIGOTROPHICUS MEDIUM

KH2PO4  0.5     g
K2HPO4  0.5     g
NH4Cl  0.5     g
NaCl  5.0     g
Na2SO4  2.84    g
MgCl2·6H2O  0.3     g
CaCl2·2H2O  0.05    g
KCl  0.4     g
Trace element solution (see Medium No. 294)  1.0     ml
Resazurin  1.0     mg
Distilled water  955.0     ml
Mix components thoroughly, adjust pH to 7.6 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  25.0     ml
1 M Sodium propionate solution  20.0     ml
Trace vitamins* (see Medium No. 197)  1.0     ml
Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add per liter the following solution (autoclaved and stored under N2):
5% Na2S·9H2O solution  8.0     ml
Readjust pH to 7.6, if necessary.