Medium data

Search for medium no. = [1144].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1144   ABYSSISOLIBACTER MEDIUM

NaCl  22.0     g
MgSO4·7H2O  3.45    g
MgCl2·6H2O  2.8     g
NH4Cl  0.5     g
KCl  0.34    
CaCl2·2H2O  0.14    g
KH2PO4  0.14    g
Trace minerals (see Medium No. 151)  1.0     ml
Trace vitamins (see Medium No. 197)  1.0     ml
Yeast extract  2.0     g
Sodium acetate·3H2O  1.4     g
FeSO4·7H2O  0.017   g
Resazurin  1.0     mg
Distilled water  955.0     ml
Mix components thoroughly and adjust pH to 7.0. Autoclave under a N2-CO2 (80:20, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter sterilized):
10% Glucose solution  5.0     ml
8% NaHCO3 solution*  40.0     ml
Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml in 60 ml serum bottles) under the same gas mixture and seal with butyl rubber stoppers. Prior to use, reduce the medium by the addition of the following solutions (per liter, autoclaved and stored under a N2 atmosphere):
5% L-Cysteine·HCl·H2O solution  10.0     ml
5% Na2S·9H2O solution  10.0     ml