Medium data

Search for medium no. = [1142].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1142 TEPIDIBACILLUS DECATURENSIS MEDIUM

CaCl₂·2H₂O	`0.08`	g
MgCl₂·6H₂O	`0.2`	g
NaCl	`10.0`	g
KCl	`1.0`	g
NH₄Cl	`1.0`	g
PIPES	`1.0`	g
Yeast extract	`0.5`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see Medium No. 187)	`1.0`	ml
Trace vitamins (see Medium No. 197)	`1.0`	ml
Selenite-tungstate solution (see Medium No. 431)	`1.0`	ml
Resazurin	`1.0`	mg
Phosphate buffer (see below)	`5.0`	ml
Distilled water	`1.0`	L

Mix components thoroughly, bring to a boil and cool down under a N₂-CO₂ (80:20, v/v) gas mixture. When the medium is cooled to around 50C, add the following components:

NaHCO₃	`2.0`	g
L-Cysteine·HCl·H₂O	`0.031`	g

Continue flushing with the gas mixture until the chemicals are dissolved completely. Distribute the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (autoclave and stored under a N₂ atmosphere):

1.0 M Glucose solution	`10.0`	ml
0.25 M Ferric citrate solution (pH 7.0)	`20.0`	ml
5% Na₂S·9H₂O solution	`0.5`	ml

Phosphate buffer:

KH₂PO₄	`12.5`	g
K₂HPO₄	`20.0`	g
Distilled water	`1.0`	L