Medium data

Search for medium no. = [1135].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


NaCl  0.5     g
NH4Cl  0.5     g
K2HPO4  0.1     g
KH2PO4  0.1     g
CaCl2·2H2O  0.1     g
MgSO4·7H2O  0.1     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Sodium formate  2.0     g
Sodium acetate  1.0     g
FeSO4·7H2O  0.002   g
L-Cysteine·HCl·H2O  0.1     g
Resazurin  0.5     mg
Distilled water  1.0     L
Mix components thoroughly, bring to a boil for several seconds, and cool down under a stream of N2-CO2 (80:20, v/v). Distribute the medium into culture vessels (e.g., 5 ml in the Hungate tube) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Add per liter the following sterile solutions from anaerobic stocks (autoclaved or filter-sterilized):
30% Tris base solution  10.0     ml
8% Na2CO3 solution  20.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Prior to inoculation, replace the gas phase with a gas mixture of H2-CO2 (80:20, v/v), and add per liter the following solution (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution  8.0     ml
Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (80:20, v/v).

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