Medium data
Search for medium no. = [1131].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1131 SALINISPIRA L21 MEDIUM
| NaCl | 60.0 | g |
| MgCl2·6H2O | 6.0 | g |
| KCl | 1.5 | g |
| Na2SO4 | 1.0 | g |
| NH4Cl | 1.0 | g |
| CaCl2·2H2O | 0.4 | g |
| K2HPO4 | 0.4 | g |
| Trace mineral solution (see Medium No. 1084) | 10.0 | ml |
| Yeast extract | 1.0 | g |
| Trypticase peptone (BD-BBL) | 1.0 | g |
| Resazurin | 0.5 | mg |
| Distilled water | 950.0 | ml |
Mix components thoroughly, bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream and autoclave under the same gas mixture. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized):
| 10% Glucose solution | 10.0 | ml |
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
| 8% NaHCO3 solution* | 30.0 | ml |
Aseptically and anaerobically, dispense the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
| 5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
Readjust pH to 7.3-7.5, if necessary.
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