Medium data
Search for medium no. = [1115].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1115 VARUNAVIBRIO MEDIUM
| NaCl | 15.0 | g |
| K2HPO4 | 0.14 | g |
| CaCl2·2H2O | 0.14 | g |
| MgSO4·7H2O | 3.4 | g |
| MgCl2·6H2O | 4.18 | g |
| KCl | 0.33 | g |
| NiCl2·6H2O | 0.5 | mg |
| Na2SeO3·5H2O | 0.5 | mg |
| Fe(NH4)2(SO4)2·6H2O | 0.01 | g |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| NH4Cl | 0.25 | g |
| Distilled water | 950.0 | ml |
Mix components thoroughly and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 15.0 | ml |
| 10% Na2S2O3·5H2O solution* | 15.0 | ml |
| 10% KNO3 solution | 20.0 | ml |
| Trace vitamins (see Medium No. 197)* | 1.0 | ml |
Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a stream of N2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Pressurize the vessels to 200 kPa with the same gas mixture.
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