Medium data

Search for medium no. = [1115].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1115   VARUNAVIBRIO MEDIUM

NaCl  15.0     g
K2HPO4  0.14    g
CaCl2·2H2O  0.14    g
MgSO4·7H2O  3.4     g
MgCl2·6H2O  4.18    g
KCl  0.33    g
NiCl2·6H2O  0.5     mg
Na2SeO3·5H2O  0.5     mg
Fe(NH4)2(SO4)2·6H2O  0.01    g
Trace minerals (see Medium No. 151)  10.0     ml
NH4Cl  0.25    g
Distilled water  950.0     ml
Mix components thoroughly and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  15.0     ml
10% Na2S2O3·5H2O solution*  15.0     ml
10% KNO3 solution  20.0     ml
Trace vitamins (see Medium No. 197)*  1.0     ml
Distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a stream of N2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Pressurize the vessels to 200 kPa with the same gas mixture.



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