Medium data
Search for medium no. = [1106].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1106 MARINE METHANOGENIC MEDIUM
| NaCl | 22.0 | g |
| CaCl2·2H2O | 0.14 | g |
| KCl | 0.34 | g |
| NH4Cl | 0.5 | g |
| MgCl2·6H2O | 2.75 | g |
| K2HPO4 | 0.14 | g |
| Yeast extract (BD-Difco) | 0.2 | g |
| Trace mineral solution (see Medium No. 1084) | 10.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 930.0 | ml |
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
| 8% NaHCO3 solution* | 30.0 | ml |
| Trace vitamins* (see Medium No. 197) | 10.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
| 5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
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