Medium data

Search for medium no. = [1106].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1106   MARINE METHANOGENIC MEDIUM

NaCl  22.0     g
CaCl2·2H2O  0.14    g
KCl  0.34    g
NH4Cl  0.5     g
MgCl2·6H2O  2.75    g
K2HPO4  0.14    g
Yeast extract (BD-Difco)  0.2     g
Trace mineral solution (see Medium No. 1084)  10.0     ml
Resazurin  1.0     mg
Distilled water  930.0     ml
Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized):
8% NaHCO3 solution*  30.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
5% Na2S·9H2O solution  10.0     ml
5% L-Cysteine·HCl·H2O solution  10.0     ml