Medium data

Search for medium no. = [1098].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1098 BSM-RC MEDIUM

NaCl	`3.62`	g
K₂HPO₄	`0.26`	g
NH₄Cl	`0.075`	g
Na₂SO₄	`0.05`	g
NaNO₃	`0.85`	g
L-Sodium lactate	`2.24`	g
Yeast extract	`0.2`	g
Tryptone	`0.5`	g
FeCl₂ solution (see Medium No. 187)	`1.0`	ml
Trace element solution (see below)	`1.0`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml
Distilled water	`935.5`	ml

Mix components thoroughly and autoclave under a N₂ atmosphere. After cooling, add the following solution (filter-sterilized):

8% NaHCO₃ solution 52.5 ml

Aseptically and anaerobically distribute the medium into culture vessels under a N₂ atmosphere and add H₂ at 5% volume of the gas phase.
Trace element solution:

MgCl₂·6H₂O	`3.0`	g
CaCl₂·2H₂O	`100.0`	mg
ZnCl₂	`70.0`	mg
MnCl₂·4H₂O	`100.0`	mg
H₃BO₃	`6.0`	mg
CoCl₂·6H₂O	`190.0`	mg
CuCl₂·2H₂O	`2.0`	mg
NiCl₂·6H₂O	`24.0`	mg
Na₂MoO₄·2H₂O	`36.0`	mg
Distilled water	`1.0`	L