Medium data

Search for medium no. = [1098].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1098   BSM-RC MEDIUM

NaCl  3.62    g
K2HPO4  0.26    g
NH4Cl  0.075   g
Na2SO4  0.05    g
NaNO3  0.85    g
L-Sodium lactate  2.24    g
Yeast extract  0.2     g
Tryptone  0.5     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see below)  1.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
Distilled water  935.5     ml
Mix components thoroughly and autoclave under a N2 atmosphere. After cooling, add the following solution (filter-sterilized):
8% NaHCO3 solution  52.5     ml
Aseptically and anaerobically distribute the medium into culture vessels under a N2 atmosphere and add H2 at 5% volume of the gas phase.
Trace element solution:
MgCl2·6H2O  3.0     g
CaCl2·2H2O  100.0     mg
ZnCl2  70.0     mg
MnCl2·4H2O  100.0     mg
H3BO3  6.0     mg
CoCl2·6H2O  190.0     mg
CuCl2·2H2O  2.0     mg
NiCl2·6H2O  24.0     mg
Na2MoO4·2H2O  36.0     mg
Distilled water  1.0     L



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