Medium data
Search for medium no. = [1095].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1095 BICARBONATE BUFFERED MEDIUM
Na2HPO4·2H2O | 0.53 | g |
KH2PO4 | 0.41 | g |
NH4Cl | 0.3 | g |
NaCl | 0.3 | g |
FeCl2 solution (see Medium No. 187) | 1.0 | ml |
Trace element solution (see Medium No. 187) | 1.0 | ml |
Selenite-tungstate solution (see Medium No. 431) | 0.5 | ml |
Yeast extract | 0.1 | g |
Resazurin | 0.5 | mg |
Distilled water | 930.0 | ml |
Mix components and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter-sterilized):
10% MgCl2·6H2O solution | 1.0 | ml |
10% CaCl2·2H2O solution | 1.0 | ml |
Trace vitamins* (see Medium No. 197) | 10.0 | ml |
8% NaHCO3 solution* | 50.0 | ml |
Substrate solution (see below) | 10.0 | ml |
Aseptically and anaerobically distribute the medium into culture vessels (e.g., 50 ml of the medium in 120 ml serum bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution | 10.0 | ml |
Substrate Solution:
For strain JCM 30818, 1 M glycerin solution;
For strain JCM 31247, 1 M trisodium citrate solution;
For strain JCM 31248, 1 M maltose solution;
For strain JCM 35360, 2 M methanol solution.
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