Medium data

Search for medium no. = [1095].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1095   BICARBONATE BUFFERED MEDIUM

Na2HPO4·2H2O  0.53    g
KH2PO4  0.41    g
NH4Cl  0.3     g
NaCl  0.3     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  0.5     ml
Yeast extract  0.1     g
Resazurin  0.5     mg
Distilled water  930.0     ml
Mix components and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter-sterilized):
10% MgCl2·6H2O solution  1.0     ml
10% CaCl2·2H2O solution  1.0     ml
Trace vitamins* (see Medium No. 197)  10.0     ml
8% NaHCO3 solution*  50.0     ml
Substrate solution (see below)  10.0     ml
Aseptically and anaerobically distribute the medium into culture vessels (e.g., 50 ml of the medium in 120 ml serum bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere):
5% Na2S·9H2O solution  10.0     ml
Substrate Solution:
For strain JCM 30818, 1 M glycerin solution;
For strain JCM 31247, 1 M trisodium citrate solution;
For strain JCM 31248, 1 M maltose solution.