Medium data
Search for medium no. = [1093].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1093 MJ BASAL MEDIUM WITH THIOSULFATE/2
MJ(-N) synthetic seawater (see Medium No. 268) | 1.0 | L |
NH4Cl | 0.25 | g |
KNO3 | 0.25 | g |
Distilled water | 1.0 | L |
Mix components thoroughly and autoclave. After cooling, add the following solutions (filter-sterilized):
8% NaHCO3 solution | 10.0 | ml |
10% Na2S2O3·5H2O solution | 15.0 | ml |
Trace vitamins (see Medium No. 197) | 1.0 | ml |
Aseptically distribute the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) and seal with butyl rubber stoppers. Replace the gas phase with a N2-CO2 (80:20, v/v) gas mixture, and pressurize to 200 kPa. Adjust pH to 6.0, if necessary.
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