Medium data
Search for medium no. = [1092].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1092 NATRONOLIMNOBIUS AHT32 MEDIUM (B)
| Soda based mineral medium (sterilized, see Medium No. 1081) | 1.0 | L |
Aseptically add per liter the following solutions (autoclaved or *filter-sterilized):
| Trace vitamins (see Medium No. 197)* | 10.0 | ml |
| 10% Yeast extract solution | 0.2 | ml |
| 1 M Sodium butyrate | 20.0 | ml |
| 1 M Sodium acetate | 20.0 | ml |
Distribute the medium into culture vessels, replace the gas phase with N2 containing 5% O2 (by vol.), and seal with butyl rubber stoppers.
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