Medium data

Search for medium no. = [1081].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1081   NATRANAEROARCHAEUM MEDIUM

Basal mineral NaCl medium (sterilized, see Medium No. 1079)  250.0     ml
Soda based mineral medium (sterilized, see below)  750.0     ml
To complete the medium, aseptically add the following solution (autoclaved or *filter-sterilized):
2 M Sodium pyruvate solution*  5.0     ml
Trace vitamins (see Medium No. 197)*  10.0     ml
10% Yeast extract solution  0.2     ml
Soda based mineral medium:
Na2CO3  185.0     g
NaHCO3  35.0     g
NaCl  16.0     g
K2HPO4  1.0     g
KCl  5.0     g
Add components to distilled water and bring volume to 1.0 L. Autoclave and let it stand for three days. Decant the clear solution into another empty sterile bottle. Aseptically add per liter the following solutions (autoclaved):
4 M NH4Cl solution  1.0     ml
Selenite-tungstate solution (see Medium No. 431)  1.0     ml
Trace element solution (see Medium No. 1079])  1.0     ml
1 M MgCl2 solution  1.0     ml