Medium data
Search for medium no. = [1090].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1090 MMJS MEDIUM 2
| NaCl | 20.0 | g |
| K2HPO4 | 0.14 | g |
| CaCl2·2H2O | 1.0 | g |
| MgSO4·7H2O | 3.4 | g |
| MgCl2·6H2O | 4.18 | g |
| NH4Cl | 0.25 | g |
| KCl | 0.33 | g |
| NiCl2·6H2O | 0.5 | mg |
| Na2SeO3·5H2O | 0.5 | mg |
| Na2WO4·2H2O | 0.1 | mg |
| (NH4)2Ni(SO4)2·6H2O | 10.0 | mg |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Distilled water | 1.0 | L |
Mix components and adjust pH to 5.5. Distribute the medium into culture vessels (e.g., 20 ml in 60 ml-serum bottles) under a stream of N2, seal with butyl rubber stoppers and add Autoclave. After cooling, add per liter the following solutions (filter-sterilized):
| Trace vitamins (see Medium No. 197) | 1.0 | ml |
| 8% NaHCO3 solution | 3.75 | ml |
| 1 M Na2S2O3 solution | 5.0 | ml |
Replace the gas phase with N2-CO2-O2 (94:5:1, v/v/v, 100 kPa). Check final pH to be around 6.2.
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