Medium data

Search for medium no. = [1090].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1090   MMJS MEDIUM 2

NaCl  20.0     g
K2HPO4  0.14    g
CaCl2·2H2O  1.0     g
MgSO4·7H2O  3.4     g
MgCl2·6H2O  4.18    g
NH4Cl  0.25    g
KCl  0.33    g
NiCl2·6H2O  0.5     mg
Na2SeO3·5H2O  0.5     mg
Na2WO4·2H2O  0.1     mg
(NH4)2Ni(SO4)2·6H2O  10.0     mg
Trace minerals (see Medium No. 151)  10.0     ml
Distilled water  1.0     L
Mix components and adjust pH to 5.5. Distribute the medium into culture vessels (e.g., 20 ml in 60 ml-serum bottles) under a stream of N2, seal with butyl rubber stoppers and add Autoclave. After cooling, add per liter the following solutions (filter-sterilized):
Trace vitamins (see Medium No. 197)  1.0     ml
8% NaHCO3 solution  3.75    ml
1 M Na2S2O3 solution  5.0     ml
Replace the gas phase with N2-CO2-O2 (94:5:1, v/v/v, 100 kPa). Check final pH to be around 6.2.