Medium data

Search for medium no. = [1066].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1066   M25Y MEDIUM

MES  3.9     g
NaCl  25.0     g
MgSO4·7H2O  2.7     g
MgCl2·6H2O  4.3     g
NH4Cl  0.25    g
KCl  0.34    g
CaCl2·2H2O  0.14    g
K2HPO4  0.14    g
Yeast extract  0.2     g
Trace elements (see Medium No. 621)  1.0     ml
0.2% Fe(NH4)2(SO4)·6H2O (in 0.037% HCl)  1.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Adjust pH to 5.5. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter sterilized):
Trace vitamins (see Medium No. 197)*  10.0     ml
5% Na2S·9H2O solution  10.0     ml



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