Medium data
Search for medium no. = [1066].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1066 M25Y MEDIUM
| MES | 3.9 | g |
| NaCl | 25.0 | g |
| MgSO4·7H2O | 2.7 | g |
| MgCl2·6H2O | 4.3 | g |
| NH4Cl | 0.25 | g |
| KCl | 0.34 | g |
| CaCl2·2H2O | 0.14 | g |
| K2HPO4 | 0.14 | g |
| Yeast extract | 0.2 | g |
| Trace elements (see Medium No. 621) | 1.0 | ml |
| 0.2% Fe(NH4)2(SO4)·6H2O (in 0.037% HCl) | 1.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Adjust pH to 5.5. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter sterilized):
| Trace vitamins (see Medium No. 197)* | 10.0 | ml |
| 5% Na2S·9H2O solution | 10.0 | ml |
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