Medium data

Search for medium no. = [621].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

621   HYL MEDIUM

HEPES  4.76    g
NaCl  20.0     g
Na2SO4  4.0     g
MgCl2·6H2O  4.3     g
NH4Cl  0.25    g
KCl  0.34    g
CaCl2·2H2O  0.14    g
K2HPO4  0.14    g
Yeast extract  1.0     g
Sodium lactate solution (50%)  4.5     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Adjust pH to 7.0.
Autoclave and cool down to room temperature while flushing with N2. Then add the following solutions from sterile anaerobic stocks:
Trace elements (see below)  1.0     ml
0.2% Fe(NH4)2(SO4)·6H2O (in 0.037% HCl)  1.0     ml
Trace vitamins (see Medium No. 197)  10.0     ml
0.5 M Na2S·9H2O solution  4.0     ml
Trace elements:
EDTA·2Na  29.0     g
MnSO4·xH2O  3.26    g
CoCl2·6H2O  1.8     g
ZnSO4·7H2O  1.0     g
NiSO4·6H2O  0.11    g
CuSO4·5H2O  0.1     g
H3BO3  0.1     g
KAl(SO4)2·12H2O  0.1     g
Na2MoO4·2H2O  0.1     g
Na2WO4·2H2O  0.1     g
Na2SeO3  0.05    g
Distilled water  1.0     L
First dissolve EDTA·2Na and adjust pH to 8.0 with KOH. Then add the minerals.



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