Medium data

Search for medium no. = [1065].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1065   ACIDIFIED MPOB MEDIUM

NH4Cl  0.3     g
Na2SO4  1.42    g
CaCl2·2H2O  0.11    g
MgCl2·6H2O  0.1     g
NaCl  0.3     g
FeCl2 solution (see Medium No. 187)  1.0     ml
Trace element solution (see Medium No. 187)  1.0     ml
Yeast extract (BD-Difco)  0.2     g
Trace vitamins (see Medium No. 197)  10.0     ml
Resazurin  0.5     mg
Distilled water  1.0     L
Mix components thoroughly, bubble with a N2-CO2 (80:20, v/v) gas mixture, seal with a butyl rubber stopper and autoclave under the same gas mixture. After cooling, aseptically and anaerobically add per liter the following solutions (separately autoclaved under a N2 atmosphere):
1 M K2HPO4 solution  5.0     ml
1 M Glycerin solution  5.0     ml
5% L-Cysteine·HCl·H2O solution  10.0     ml



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