Medium data
Search for medium no. = [1065].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1065 ACIDIFIED MPOB MEDIUM
| NH4Cl | 0.3 | g |
| Na2SO4 | 1.42 | g |
| CaCl2·2H2O | 0.11 | g |
| MgCl2·6H2O | 0.1 | g |
| NaCl | 0.3 | g |
| FeCl2 solution (see Medium No. 187) | 1.0 | ml |
| Trace element solution (see Medium No. 187) | 1.0 | ml |
| Yeast extract (BD-Difco) | 0.2 | g |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Resazurin | 0.5 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly, bubble with a N2-CO2 (80:20, v/v) gas mixture, seal with a butyl rubber stopper and autoclave under the same gas mixture. After cooling, aseptically and anaerobically add per liter the following solutions (separately autoclaved under a N2 atmosphere):
| 1 M K2HPO4 solution | 5.0 | ml |
| 1 M Glycerin solution | 5.0 | ml |
| 5% L-Cysteine·HCl·H2O solution | 10.0 | ml |
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