Medium data
Search for medium no. = [1062].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1062 DESULFUROMONAS CARBONIS MEDIUM
| NaCl | 8.8 | g |
| MgCl2·6H2O | 4.6 | g |
| KCl | 0.34 | g |
| NH4Cl | 0.25 | g |
| KH2PO4 | 0.14 | g |
| CaCl2·2H2O | 0.14 | g |
| NiCl2·6H2O | 9.3 | mg |
| Fe(NH4)2(SO4)2·6H2O | 2.7 | mg |
| Na2SeO3·5H2O | 0.26 | mg |
| Na2WO4·2H2O | 0.084 | mg |
| Trace element solution (see Medium No. 187) | 10.0 | ml |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Disodium fumarate | 8.0 | g |
| Distilled water | 1.0 | L |
Mix components thoroughly, distribute the medium into culture vessels (e.g., 10 ml medium in Balch-type tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions:
| 8% NaHCO3 solution (filter-sterilized) | 60.0 | ml |
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