Medium data
Search for medium no. = [1057].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
1057 ACETOMICROBIUM FAECALE MEDIUM
| Trypticase peptone (BD-BBL) | 2.0 | g |
| Yeast extract | 2.0 | g |
| Glucose | 4.0 | g |
| KH2PO4 | 0.225 | g |
| K2HPO4 | 0.255 | g |
| (NH4)2SO4 | 0.255 | g |
| NaCl | 0.5 | g |
| MgSO4·7H2O | 0.1 | g |
| CaCl2·2H2O | 0.07 | g |
| Sodium acetate·3H2O | 5.0 | g |
| Trace vitamins (see Medium No. 197) | 10.0 | ml |
| Trace minerals solution (see below) | 10.0 | ml |
| Resazurin | 1.0 | mg |
| Distilled water | 1.0 | L |
Mix components thoroughly. Bring to a boil for seconds, cool down under a N2-CO2 (4:1, v/v) gas mixture and add the following components to the medium:
| NaHCO3 | 6.0 | g |
| L-Cysteine·HCl·H2O | 0.5 | g |
Seal with butyl rubber stoppers and autoclave. After inoculation, pressurize the inoculated bottles to 200 kPa with a N2-CO2 (4:1, v/v) gas mixture.
Trace minerals solution:
| Trace minerals (see Medium No. 151) | 1.0 | L |
| NiCl2·6H2O | 0.03 | g |
| Na2SeO3·5H2O | 0.3 | mg |
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