Medium data

Search for medium no. = [1057].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


Trypticase peptone (BD-BBL)  2.0     g
Yeast extract  2.0     g
Glucose  4.0     g
KH2PO4  0.225   g
K2HPO4  0.255   g
(NH4)2SO4  0.255   g
NaCl  0.5     g
MgSO4·7H2O  0.1     g
CaCl2·2H2O  0.07    g
Sodium acetate·3H2O  5.0     g
Trace vitamins (see Medium No. 197)  10.0     ml
Trace minerals solution (see below)  10.0     ml
Resazurin  1.0     mg
Distilled water  1.0     L
Mix components thoroughly. Bring to a boil for seconds, cool down under a N2-CO2 (4:1, v/v) gas mixture and add the following components to the medium:
NaHCO3  6.0     g
L-Cysteine·HCl·H2O  0.5     g
Seal with butyl rubber stoppers and autoclave. After inoculation, pressurize the inoculated bottles to 200 kPa with a N2-CO2 (4:1, v/v) gas mixture.
Trace minerals solution:
Trace minerals (see Medium No. 151)  1.0     L
NiCl2·6H2O  0.03    g
Na2SeO3·5H2O  0.3     mg

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