Medium data

Search for medium no. = [1017].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1017 PFENNIG THERMOFILUM MEDIUM

NH₄Cl	`0.33`	g
KH₂PO₄	`0.33`	g
KCl	`0.33`	g
CaCl₂·2H₂O	`0.33`	g
MgCl₂·6H₂O	`0.33`	g
Trace element solution (see Medium No. 899)	`1.0`	ml
Trace vitamins (see Medium No. 197)	`10.0`	ml
Glucose	`2.0`	g
Yeast extract (Oxoid)	`0.1`	g
Trypticase peptone (BD-BBL)	`0.5`	g
Archaeal culture filtrate (see below)	`10.0`	ml
Resazurin	`1.0`	mg
Distilled water	`1.0`	L

Mix components thoroughly, adjust pH to 6.0 and autoclave under a N₂ gas atmosphere. Before inoculation, add per liter the following solution (autoclaved and stored under a N₂ gas atmosphere):

5% Na₂S·9H₂O solution (neutralized) 6.0 ml

Archaeal culture filtrate:
Cultivate Desulfurococcus kamchatkensis strain 1221n (=JCM 16383) in the above described medium supplemented with 0.6 g/L (final) yeast extract (without the archaeal culture filtrate). After three days of the cultivation, filtrate the grown culture with 0.22 µm (pore size) filter.
Comment: For cultivation of JCM 19810, increase amount of yeast extract to 1.0 g/L. Growth may be affected by quality of yeast extract.