Medium data

Search for medium no. = [1012].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.


UBS solution (see Medium No. 729)  100.0     ml
Trace minerals (see Medium No. 151)  10.0     ml
Ni-Se-W solution (see Medium No. 244)  10.0     ml
Glucose  0.9     g
Yeast extract  0.2     g
Distilled water  1.0     L
Mix components thoroughly, adjust pH 2.5 with H2SO4 and autoclave. After cooling, add the following solution (filter-sterilized) to the medium:
1 M FeSO4 solution (pH 2.0)  5.0     ml