Medium data
Search for medium no. = [729].
Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.
729 ACIDITHIOBACILLUS FERRIVORANS MEDIUM
| UBS solution (see below) | 100.0 | ml |
| Trace minerals (see Medium No. 151) | 10.0 | ml |
| Ni-Se-W solution (see Medium No. 244) | 10.0 | ml |
| Distilled water | 845.0 | ml |
Mix thoroughly, adjust pH to 2.5 with H2SO4 and autoclave. After cooling, add the following solutions (filter-sterilized) to the medium:
| 0.1 M K2S4O6 solution | 25.0 | ml |
| 10 mM FeSO4 solution (pH 2.0) | 10.0 | ml |
UBS solution:
| Na2SO4 | 14.0 | g |
| (NH4)2SO4 | 30.0 | g |
| KCl | 1.0 | g |
| MgSO4·7H2O | 5.0 | g |
| K2HPO4 | 0.5 | g |
| Ca(NO3)2·4H2O | 0.14 | g |
| Distilled water | 1.0 | L |
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