Medium data

Search for medium no. = [1009].

Unless otherwise stated, sterilize media by autoclaving at 121°C for 15 min.

1009 CLOSTRIDIUM SWELLFUNIANUM MEDIUM

NH₄Cl	`0.33`	g
KCl	`0.33`	g
KH₂PO₄	`0.33`	g
Yeast extract (BD-Difco)	`0.5`	g
Tryptone (BD-Difco)	`3.0`	g
Trace element solution SL-10 (see Medium No. 433 )	`1.0`	ml
Trace vitamins (see Medium No. 197 )	`10.0`	ml
1% CaCl₂·2H₂O solution	`33.0`	ml
2% MgCl₂·6H₂O solution	`16.5`	ml
L-Cysteine·HCl·H₂O	`0.5`	g
1% Resazurin solution	`1.0`	ml
Distilled water	`1.0`	L

Adjust pH to 7.3.
Prepare the medium anaerobically under a N₂-CO₂ (4:1, v/v) gas mixture. Distribute the medium into culture vessels (e.g., 5.0 ml in Hungate tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave. After autoclaving, add per 5.0 ml the following solutions from anaerobic stocks (autoclaved or *filter-sterilized and stored under a N₂ atmosphere):

3% (w/v) Na₂S·9H₂O solution	`0.05`	ml
10% (w/v) NaHCO₃ solution*	`0.05`	ml
20% (w/v) Glucose solution	`0.1`	ml